Stephen Bell published in the scientific-journal Cell: Folder Name: News & Events: Interdisciplinary Biochemistry Program: Indiana University Bloomington

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Stephen Bell published in the scientific-journal Cell

Stephen Bell published in the scientific-journal Cell

Wednesday, September 25, 2019

Graphical Abstract from "Physical and Functional Compartmentalization of Archaeal Chromosomes" Naomichi Takemata, Rachel Y.Samson, Stephen D.Bell

The labs of Stephen Bell and Rachel Y. Samson have published a new manuscript in the journal Cell. The article, entitled "Physical and Functional Compartmentalization of Archaeal Chromosomes”, reveals hitherto unrecognized similarities between the organization of archaeal chromosomes and those of humans. The paper also describes the identification of a novel chromosome-organizing protein termed “Coalescin (ClsN)".

Coalescin, a Novel Sulfolobus-Encoded SMC Protein

(A) Phyletic distribution of ClsN. The schematic phylogenetic tree is depicted according to

Spang et al. (2017) See STAR Methods for details.

(B) Model for ClsN structure generated using the Phyre 2 server.

(C) Western blot analyses correlating molar amounts of ClsN protein from specific numbers of cells to defined amounts of recombinant proteins. Analyses were performed with both *S. islandicus* extract and recombinant protein and *S. acidocaldarius* extract and recombinant protein. Calibration curves appear in the right-hand panels. Quantitation was performed using ImageJ.

(D) Analyses on ClsN peaks in *S. acidocaldarius* (Sac). Left panel: Width distribution of ClsN peaks. Middle panel: Scatterplot comparing peak width and enrichment of ClsN. Right panel: Boxplots comparing ClsN enrichment in protein-coding genes and insertion sequences (ISs). All data points were additionally plotted for the ISs because of their small number (yellow diamonds). p-values were calculated by Wilcoxon rank sum test.

(E) The same analyses as in panel (D) were conducted on ClsN peaks in *S. islandicus* (Sis).

To read the entire article "Physical and Functional Compartmentalization of Archaeal Chromosomes", visit the scientific-journal Cell article:

Read the Cell article

Author Contributions

Naomichi Takemata, Rachel Y.Samson, and Stephen D.Bell designed and performed experiments, analyzed data, and wrote the manuscript.

More About

Stephen Bell

Biochemistry Graduate Program