Biochemical and Genetic Analysis of the Chlamyida GroEL Chaperonins

Melissa Illingworth, Anna J. Hooppaw, Lu Ruan, Derek Fisher , Lingling Chen
Publication Date
June 2017
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Abstract

Chaperonins are essential for cellular growth under normal and stressful conditions and consequently represent one of the most conserved and ancient protein classes. The paradigm Escherichia coli chaperonin, EcGroEL, and its cochaperonin, EcGroES, assist in the folding of proteins via an ATP-dependent mechanism. In addition to the presence of groEL and groES homologs, groEL paralogs are found in many bacteria, including pathogens, and have evolved poorly understood species-specific functions. Chlamydia spp., which are obligate intracellular bacteria, have reduced genomes that nonetheless contain three groEL genes, Chlamydia groEL (ChgroEL), ChgroEL2, and ChgroEL3.

Citation

Melissa Illingworth, Anna J. Hooppaw, Lu Ruan, Derek Fisher and Lingling Chen. Biochemical and Genetic Analysis of the Chlamyida GroEL Chaperonins. J. Bacteriol. June 2017 vol. 199 no. 12 e00844-16